Salient effects of l-carnitine on adenine-nucleotide loss and coenzyme A acylation in the diabetic heart perfused with excess palmitic acid: A phosphorus-31 NMR and chemical extract study |
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| Authors: | Galen M Pieper Wallace J Murray JM Salhany Shao T Wu Robert S Eliot |
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| Institution: | 1. Department of Preventive and Stress Medicine, 3012 Poynter Hall, Omaha, NE 68105 U.S.A.;2. Department of Biomedicinal Chemistry, University of Nebraska Medical Center, Omaha, NE 68105 U.S.A.;3. VA Medical Center, Omaha, NE 68105 U.S.A. |
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| Abstract: | The beneficial effects of l-carnitine perfusion on energy metabolism and coenzyme A acylation were studied in isolated hearts from control and diabetic rats. All hearts were perfused at a constant flow rate with a glucose/albumin buffer which contained 2.0 mM palmitate. 31P-NMR was utilized to assess sequential phosphocreatine and ATP metabolism during 1 h of recirculation perfusion. l-Carnitine (5.0 mM final concentration) was added after 12 min of baseline recirculation perfusion. Frozen samples were taken after 1 h of recirculation perfusion for spectrophotometric analysis of high-energy phosphates and the free and acylated fractions of coenzyme A. l-Carnitine perfusion of diabetic hearts attenuated or prevented the reduction of ATP observed in untreated diabetic hearts. It also attenuated the accumulation of long-chain fatty-acyl coenzyme A. Although l-carnitine improved myocardial function in diabetic hearts, this was independent of any direct effect on physiological indices. Thus, the salutory effect of acute perfusion with l-carnitine on energy metabolism in the isolated perfused diabetic rat heart appears to be a direct effect on lipid metabolism. |
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| Keywords: | L-Carnitine Long-chain acyl-CoA Diabetes ATP (Rat heart) |
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