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Sensitivity of ATPase-ADPase activities from synaptic plasma membranes of rat forebrain to lipid peroxidation in vitro and the protective effect of vitamin E |
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| Authors: | Marion Vietta Silvana S. Frassetto Ana M. O. Battastini Adriane Bello-Klein Cleci Moreira Renato D. Dias Jao J. F. Sarkis |
| Affiliation: | (1) Departmento de Bioquimica, Instituto de Biociências, Universidadé Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS, Brasil;(2) Departmento de Fisiologia, Laboratório de Fisiologia Cardiovascular, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS, Brasil |
| Abstract: | The in vitro effects of membrane lipid peroxidation on ATPase-ADPase activities in synaptic plasma membranes from rat forebrain were investigated. Treatment of synaptic plasma membranes with an oxidant generating system (H2O2/Fe2+/ascorbate) resulted in lipid peroxidation and inhibition of the enzyme activity. Besides, trolox as a water soluble vitamin E analogue totally prevented lipid peroxidation and the inhibition of enzyme activity. These results demonstrate the susceptibility of ATPase-ADPase activities of synaptic plasma membranes to free radicals and suggest that the protective effect against lipid peroxidation by trolox prevents the inhibition of enzyme activity. Thus, inhibition of ATPase-ADPase activities of synaptic plasma membranes in cerebral oxidative stress probably is related to lipid peroxidation in the brain. |
| Keywords: | ATPase-ADPase ATP diphosphohydrolase free radicals oxidative stress synaptic plasma membranes |
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