Production of recombinant hIL-4delta2-a native isoform of human interleukin-4 in Escherichia coli cells |
| |
| Authors: | Ptitsyn L R Smirnov S V Al'tman I B Samsonova N N Khodiakova A V Vasilenko R N |
| |
| Institution: | Russian Federation State Research Center Genetika, Moscow, Russia. mashko@vnigen.msk.su |
| |
| Abstract: | Expression plasmids containing the synthetic gene hil-4 delta 2 was constructed to produce human interleukin-4 in Escherichia coli cells. Strains TG1 (pBTIL-4 delta 2) and BL21 (DE3) (pETIL-4 delta 2) produced the recombinant protein as inclusion bodies, and its production level was up to 30% of the total cell protein. The renatured hIL-4 delta 2 inhibited IL-4-stimulated T cell proliferation, and this effect was enhanced by cyclosporin A. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
