Interaction of ceruloplasmin and 5-lipoxygenase |
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Authors: | A V Sokolov E A Golenkina V A Kostevich V B Vasilyev G F Sud’ina |
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Institution: | Research Institute of Experimental Medicine, St. Petersburg 197376, Russia. biochemsokolov@gmail.com |
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Abstract: | The interaction between ceruloplasmin (CP), the multicopper oxidase of human plasma, and 5-lipoxygenase (5-LO), the key enzyme
of leukotriene synthesis, is shown for the first time. By Western-blotting and mass spectrometry of tryptic fragments, it
is shown that 5-LO from protein extract of human leukocytes binds with immobilized CP. Dose-dependent influence of intact
CP on leukotrienes synthesis is found: CP reduced leukotrienes synthesis in leukocytes in a dose above 50 μg/ml (normal CP
concentration in plasma is about 300–400 μg/ml). Proteolyzed CP and apo-form of CP is unable to inhibit activity of 5-LO.
CP increased activity of 5-LO at low doses (5–10 μg/ml). On the whole, the influence of CP on phagocytosis index of leukocytes
coordinates with influence on activity of 5-LO: the index increased in the range of 2–10 μg/ml CP and decreased at doses of
CP above 40 μg/ml. The dual role of CP in regulation of cellular response of leukocytes is discussed. |
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