葡萄花药三倍体植株的倍性鉴定

用卡宝品红染色法检查格列那什二倍体葡萄(Vitis vinifera var. Cre nache)花药再生植株的染色体, 并用UNICHRO软件对其数目及长度进行了测量。结果表明,这些花药植株根尖中染色体组型为2n=3x=57的细胞平均占观察细胞总数的86.5%,来自不同花药的再生殖株均为三倍体。讨论了这些植株的可能来源。 Abstrart:The chromosome number in the root tip of triploid plants regenerated from anther culture of diploid Vitis vinifera var.Grenache were examined with Carbolfuchsin staining method and the length of chromosomes were measured with UNICHRO package.The results show that average pecentage of cells which have a karyotype for 2n=3x=57 in root tip of pollen plants were 86.5% in the total number of cells observed,the plants regenerated from different anthers are triploid.Their possible origin is discussed.

Appraisal of Ploidy for Triploid Plants Regenerated from Anther Culture of Vitis vinifera var. Grenache

The chromosome number in the root tip of triploid plants regenerated from anther culture of diploid Vitis vinifera var. Grenache were examined with Carbolfuchsin staining method and the length of chromosomes were measured with UNICHRO package. The results show that the average pecentage of cells which have a karyotype for 2n= 3x = 57 in root tip of pollen plants were 86.5% in the total number of cells observcd, the plants rcgenerated from different anthers are triploid. Their possible origin is discussed.