Stimulation of Rg3 ginsenoside biosynthesis in ginseng hairy roots elicited by methyl jasmonate |
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Authors: | Ok Tae Kim Nam Hee Yoo Gum Soog Kim Young Chang Kim Kyong Hwan Bang Dong Yun Hyun Seung Hye Kim Min Young Kim |
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Institution: | 1. Planning and Coordination Division, National Institute of Horticultural & Herbal Science, RDA, Suwon, 440-706, South Korea 2. Industrial Cooperation Foundation, Chonbuk National University, Jeonju, 561-756, South Korea 3. Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 369-871, South Korea 4. Medicinal Crop Seed Supply Center, Jeollanamdo Development Institute for Traditional Korean Medicine, Jangheung, 529-851, South Korea
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Abstract: | It has been recognized that ginsenoside Rg3 is not naturally produced in ginseng although this ginsenoside can accumulate in red ginseng as the result of a thermal process. In order to determine whether or not Rg3 is synthesized in ginseng, hairy roots were treated with methyl jasmonate (MJ). From HPLC analysis, no peak for Rg3 was observed in the controls. However, Rg3 did accumulate in hairy roots that were MJ-treated for 7?days. Rg3 content was 0.42?mg/g (dry weight). To gain more insight into the effects of MJ on UDP-glucosyltransferase (UGT) activity, we attempted to evaluate ginsenoside Rg3 biosynthesis by UGT. A new peak for putative Rg3 was observed, which was confirmed by LC-MS/MS analysis. Our findings indicate that the proteins extracted from our hairy root lines can catalyze Rg3 from Rh2. This suggests that our ginseng hairy root lines possess Rg3 biosynthesis capacity. |
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