Sugarcane tissue and protoplast culture |
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Authors: | P J Larkin |
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Institution: | (1) Division of Plant Industry, CSIRO, PO Box 1600, 2601 Canberra City, ACT, Australia |
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Abstract: | Experiments are described which improve the protocols for initiating in vitro cultures of sugarcane and allowing efficient
regeneration of plants even after 30 months of callus proliferation. Procedures adopted included use of leaf base explants,
CS medium with 3 mg/l 2, 4-D and 0.25 mg/l kinetin for callus initiation and growth, MS medium with 0.5 mg/l IAA and 1 mg/l
BAP for shoots, MS medium with 5 mg/l NAA and 7% (wt/vol) sucrose for rooting of shoots. Casein hydrolysate (N-Z amine) significantly
shortened the lag period in the growth of sugarcane suspension cultures, but did not increase the rate of growth following
the lag phase. Protoplasts isolated from two types of cultures could be grown to re-establish cell cultures but no plants
have yet been regenerated derived from isolated protoplasts. |
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Keywords: | Saccharum cell culture protoplasts embryogenesis |
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