Rapid plant regeneration from callus cultures of Plumbago zeylanica |
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Authors: | Rout GR Saxena C Samantaray S Das P |
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Institution: | (1) Plant Biotechnology Division, Plant Tissue Culture Laboratory Regional Plant Resource Centre, Bhubaneswar, 751015 Orissa, (India);(2) Plant Physiology and Biochemistry Laboratory, Regional Plant Resource Centre, Bhubaneswar, 751015 Orissa, (India) |
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Abstract: | Rapid plant regeneration was achieved in callus cultures derived from leaf and stem explants of Plumbago zeylanica Linn. on MS basal medium supplemented with 4.44 M 6-BA, 1.42 M IAA and 3% (w/v) sucrose. The rate of shoot bud regeneration was positively correlated with the concentration of growth regulators in the nutrient media. The leaf explants were more responsive (82.3%) than the stem explants on medium containing 1.42M IAA in combination with 4.44 M BA. The rate of regeneration was found to maintain the same level for 12 months without loss of vigour. Rooting of the differentiated shoots was achieved in media having 0.57 M IAA with 2% (w/v) sucrose within 10 days of culture. Regenerated plantlets were transferred to soil which grew normally with a survival rate of 90%. This protocol may help in the conservation of the species and selection of variants that may be induced to widen the genetic base of the genus. |
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Keywords: | growth regulators organogenesis regeneration medicinal plants |
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