Somatic embryogenesis and regeneration of plants in the bamboo Dendrocalamus strictus |
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Authors: | Rao I Usha Ramanuja Rao I V Narang Vibha |
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Institution: | (1) Department of Botany, University of Delhi, 110 007 Delhi, India |
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Abstract: | Somatic embryogenesis leading to plant regeneration has been achieved in the bamboo, Dendrocalamus strictus, by culturing seeds (caryopses) on B5 basal medium supplemented with 2,4-dichlorophenoxyacetic acid. Callus cultures obtained from the embryonal end of the seeds differentiated chlorophyllous embryoids. On transfer to a germination medium (B5 liquid, sucrose, indolebutyric acid, and -naphthaleneacetic acid) 40% of the embryoids developed into plantlets. Further development of the plantlets occured on B5 liquid medium (half strength) + sucrose (1%) + IBA (5 × 10–7M) + NAA (10–7M).Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- IBA
Indolebutyric acid
- NAA
-naphthaleneacetic acid |
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Keywords: | Bamboo Dendrocalamus strictus Regeneration Somatic Embryogenesis |
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