微波对阿维拉霉素产生菌诱变效应的研究

阿维拉霉素产生菌SV微波诱变效应的研究发现：菌株SV对微波敏感（微波处理60s，SV菌株存活率低于10％），菌落形态变化大。微波诱变处理最佳作用方式为培养皿不加盖、快速冰上冷却，最佳处理时间为50s（其正突变率25．3％）。通过微波诱变处理、阿维拉霉素推理筛选，最终获得一株稳定性良好，阿维拉霉素产量达到21．5mg／L，较出发菌株提高119．4％的突变株SV-15。

A Study on Mutagenic effects of Avilamycin-producing Strain Induced by Microwave Irradiation

To investigate Mutagenic effects of Avilamycin-producing Strain induced by microwave irradiation.The experimental results indicated that the capacity of SV strain to microwave irradiation was sensitive(its survival rate was under 10% for 60s radiation).Not sealing the culture dish and fast cooling it was the optimal irradiation mode of microwave,and 50 seconds was the optimal irradiation time of microwave(positive mutation rate,25.3%).To obtain high-yield avilamycin-producing strain,50s microwave irradiation and screening of avilamycin-rational mutant is used for the study on screening mutation.SV-15 mutants were obtained,and the mutant could produce 21.5mg/L avilamycin,which was 2.194 times as much as the potency of original strain by flake fermentation.