Effect of Mouse Strain in a Model of Chemical-induced Respiratory
Allergy |
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Authors: | Risako Nishino Tomoki Fukuyama Yuko Watanabe Yoshimi Kurosawa Hideo Ueda Tadashi Kosaka |
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Institution: | The Institute of Environmental Toxicology, Uchimoriya-machi 4321, Joso-shi, Ibaraki 303-0043, Japan |
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Abstract: | The inhalation of many types of chemicals is a leading cause of allergic respiratory
diseases, and effective protocols are needed for the detection of environmental
chemical–related respiratory allergies. In our previous studies, we developed a method for
detecting environmental chemical–related respiratory allergens by using a long-term
sensitization–challenge protocol involving BALB/c mice. In the current study, we sought to
improve our model by characterizing strain-associated differences in respiratory allergic
reactions to the well-known chemical respiratory allergen glutaraldehyde (GA). According
to our protocol, BALB/c, NC/Nga, C3H/HeN, C57BL/6N, and CBA/J mice were sensitized
dermally with GA for 3 weeks and then challenged with intratracheal or inhaled GA at 2
weeks after the last sensitization. The day after the final challenge, all mice were
euthanized, and total serum IgE levels were assayed. In addition, immunocyte counts,
cytokine production, and chemokine levels in the hilar lymph nodes (LNs) and
bronchoalveolar lavage fluids (BALF) were also assessed. In conclusion, BALB/c and NC/Nga
mice demonstrated markedly increased IgE reactions. Inflammatory cell counts in BALF were
increased in the treated groups of all strains, especially BALB/c, NC/Nga, and CBA/J
strains. Cytokine levels in LNs were increased in all treated groups except for C3H/HeN
and were particularly high in BALB/c and NC/Nga mice. According to our results, we suggest
that BALB/c and NC/Nga are highly susceptible to respiratory allergic responses and
therefore are good candidates for use in our model for detecting environmental chemical
respiratory allergens. |
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Keywords: | glutaraldehyde repiratory allergy strain difference |
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