Ly6Clow and Not Ly6Chigh Macrophages Accumulate First in the Heart in a Model of Murine Pressure-Overload |
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Authors: | Christina Weisheit Yunyang Zhang Anton Faron Odilia K?pke Gunnar Weisheit Arne Steinstr?sser Stilla Frede Rainer Meyer Olaf Boehm Andreas Hoeft Christian Kurts Georg Baumgarten |
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Institution: | 1. Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn, Bonn, Germany.; 2. Geschwister-Scholl-Gymnasium, Academic High School Daun, Daun, Germany.; 3. Institute of Physiology II, University of Bonn, Bonn, Germany.; 4. Institute of Experimental Immunology, University Hospital Bonn, Bonn, Germany.; Albert Einstein College of Medicine, United States of America, |
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Abstract: | Cardiac tissue remodeling in the course of chronic left ventricular hypertrophy requires phagocytes which degrade cellular debris, initiate and maintain tissue inflammation and reorganization. The dynamics of phagocytes in left ventricular hypertrophy have not been systematically studied. Here, we characterized the temporal accumulation of leukocytes in the cardiac immune response by flow cytometry and fluorescence microscopy at day 3, 6 and 21 following transverse aortic constriction (TAC). Cardiac hypertrophy due to chronic pressure overload causes cardiac immune response and inflammation represented by an increase of immune cells at all three time points among which neutrophils reached their maximum at day 3 and macrophages at day 6. The cardiac macrophage population consisted of both Ly6Clow and Ly6Chigh macrophages. Ly6Clow macrophages were more abundant peaking at day 6 in response to pressure overload. During the development of cardiac hypertrophy the expression pattern of adhesion molecules was investigated by qRT-PCR and flow cytometry. CD11b, CX3CR1 and ICAM-1 determined by qRT-PCR in whole cardiac tissue were up-regulated in response to pressure overload at day 3 and 6. CD11b and CX3CR1 were significantly increased by TAC on the surface of Ly6Clow but not on Ly6Chigh macrophages. Furthermore, ICAM-1 was up-regulated on cardiac endothelial cells. In fluorescence microscopy Ly6Clow macrophages could be observed attached to the intra- and extra-vascular vessel-wall. Taken together, TAC induced the expression of adhesion molecules, which may explain the accumulation of Ly6Clow macrophages in the cardiac tissue, where these cells might contribute to cardiac inflammation and remodeling in response to pressure overload. |
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