Characterization of Neurospora crassa polyadenylated messenger ribonucleic acid: structure of the 5'-terminus. |
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| Authors: | B L Seidel E W Somberg |
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| Affiliation: | The Biochemistry Laboratory, Department of Zoology and Physiology, Rutgers, The State University of New Jersey, Newark, New Jersey 07102 U.S.A. |
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| Abstract: | ![]()
Polyadenylated (poly(A)+) mRNA from Neurospora crassa was isolated by affinity chromatography on poly(U) Sepharose and its structure was examined. Two 5′-terminal ·cap’ structures, m7G(5′)ppp(5′)Ap and m7G(5′)ppp(5′)Gp, occurring in a relative distribution of 75 and 25% were found. No evidence was obtained for 2′-O-methylation in a nucleotide adjacent to the 5′-terminal cap. |
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