Cloning and characterization of rhesus monkey MCH-R1 and MCH-R2 |
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Authors: | Fried Steven O'Neill Kim Hawes Brian E |
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Institution: | Schering-Plough Research Institute, CNS/CV Biological Research, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. |
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Abstract: | Rhesus monkey MCH-R1 and MCH-R2 receptors were cloned. Amino acid homology is 98.8% between monkey and human MCH-R1, while monkey and human MCH-R2 are 98% homologous. Binding and intracellular signaling characteristics of the monkey receptors were compared with the human homologues. The results demonstrate that MCH binds to the monkey MCH-R1 receptor with a K(d) of 6.5 nM and monkey MCH-R2 with a K(d) of 2.2 nM similar to K(d) values for human MCH-R1 and MCH-R2. Additionally, monkey MCH-R1 couples through G(i)/G(o) and G(q)-type G proteins similar to human MCH-R1 whereas monkey and human MCH-R2 utilize the G(q) signaling pathway. |
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