Production of bacterial cellulose from <Emphasis Type="Italic">Enterobacter amnigenus</Emphasis> GH-1 isolated from rotten apple |
| |
Authors: | Basavaraj S Hungund S G Gupta |
| |
Institution: | (1) Department of Biotechnology, B.V.B. College of Engineering and Technology, Vidyanagar, Hubli, Karnataka, 580031, India;(2) State Institute for Administrative Careers, Hazarimal Somani Marg, Near CST, Mumbai, India |
| |
Abstract: | Bacterial cellulose finds novel applications in biomedical, biosensor, food, textile and other industries. The optimum fermentation
conditions for the production of cellulose by newly isolated Enterobacter amnigenus GH-1 were investigated. The strain was able to produce cellulose at temperature 25–35°C with a maximum at 28°C. Cellulose
production occurred at pH 4.0–7.0 with a maximum at 6.5. After 14 days of incubation, the strain produced 2.5 g cellulose/l
in standard medium whereas cellulose yield in the improved medium was found to be 4.1 g/l. The improved medium consisted of
4% (w/v) fructose, 0.6% (w/v) casein hydrolysate, 0.5% (w/v) yeast extract, 0.4% (w/v) disodium phosphate, and 0.115% (w/v)
citrate. Addition of metal ions like zinc, magnesium, and calcium and solvents like methanol and ethanol were found to be
stimulatory for cellulose production by the strain. The strain used natural carbon sources like molasses, starch hydrolysate,
sugar cane juice, coconut water, coconut milk, pineapple juice, orange juice, and pomegranate juice for growth and cellulose
production. Fruit juices can play important role in commercial exploitation of bacterial cellulose by lowering the cost of
the production medium. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|