Erk is involved in the differentiation induced by diallyl disulfide in the human gastric cancer cell line MGC803 |
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Authors: | Hui Ling Liang-Yun Zhang Qi Su Ying Song Zhao-Yang Luo Xiu Tian Zhou Xi Zeng Jie He Hui Tan Jing-Ping Yuan |
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Institution: | (1) Cancer Research Institute, Nanhua University, Hengyang City, Hunan Province, 421001, China |
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Abstract: | Diallyl disulfide (DADS) is a major constituent of garlic. Previously, we found that DADS both inhibited proliferation in
human gastric cancer cells in vitro and in vivo, and induced G2/M arrest. In this study, we investigated whether this differentiation effect was induced by DADS in human
gastric cancer MGC803 cells, and whether it was related to an alteration in ERK activity. The results showed that the growth
of MGC803 cells was inhibited by DADS. Cells treated with DADS displayed a lower nucleocytoplasmic ratio and tended to form
gland and intercellular conjunction structures. The ConA-mediated cell agglutination ratio and cells’ ALP specific activity
decreased. In MGC803 cells, dye transfer was limited to a few cells neighbouring the dye-injected cell and to a depth of 1–2
layers beneath the scrape site. However, after treatment with DADS, the LY (Lucifer Yellow) was transferred to several cells
immediately neighbouring the microinjected cell and to a depth of 2–4 cell layers from the scrape site. This indicated that
DADS induced differentiation in MGC803 cells. Western blot analysis revealed that although DADS did not influence the quantity
of ERK1/2 protein expressed, it did decrease its phosphorylation in a concentration-dependent manner, compared with the controls.
At 30 mg·L−1, DADS inhibited the activation of ERK1/2 in 15–30 min. These results suggested that the DADS-induced differentiation of MGC803
cells involved an alteration of the ERK1/2 signaling pathway. |
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Keywords: | Diallyl disulfide Stomach neoplasm Differentiation ERK |
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