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Transformation of the wild tomatoLycopersicon chilense Dun. byAgrobacterium tumefaciens
Authors:Zahra Agharbaoui  Ann Francine Greer  Zohreh Tabaeizadeh
Affiliation:(1) Department of Biological Sciences, University of Quebec in Montreal, Station "ldquo"Centre-ville"rdquo", P.O. Box 8888, H3C 3P8 Montreal, QC, Canada
Abstract:
Summary Leaf disc transformation-regeneration technique was applied to the drought tolerant wild relative of cultivated tomato,Lycopersicon chilense, using a plasmid construct which contained the coding sequences of neomycin phosphotransferase (NPTII) and chloramphenicol acetyltransferase (CAT) genes. The two genotypes used, LA2747 and LA1930, showed a distinct difference in their aptitude to transformation; a higher success rate was obtained for the first genotype in every stage of the process. Shoots were formed on the regeneration medium containing 100 mgrg/ml kanamycin through direct or indirect organogenesis. Root formation became only possible when the concentration of kanamycin was reduced to 50 mgrg/ml. Expression of chloramphenicol acetyltransferase gene was observed in all of the kanamycin-screened plants after they matured; the activity of the gene was absent or low in some of the young plants. The presence of the CAT gene in transgenic plants was further confirmed by Southern blot analysis. Although transgenic plants grew to maturity, they did not produce fruit, owing to the self incompatibility ofL. chilense.Abbreviations BAP 6-benzylaminopurine - CAT chloramphenicol acetyltransferase - 2,4-D 2,4-dichlorophenoxyacetic acid - IAA indole-3-acetic acid - LB Luria Broth - EDTA ethylenediamine-tetraacetic acid
Keywords:Agrobacterium tumefaciens  Lycopersicon chilense  Regeneration  Transformation
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