On the composition of the nucleolus with special reference to its filamentous structure |
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Authors: | Sibdas Ghosh Renate Lettré Ira Ghosh |
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Institution: | (1) Institut für experimentelle Krebsforschung des Deutschen Krebsforschungszentrums Heidelberg, Deutschland;(2) Present address: Department of Botany, Calcutta University, 35, Ballygunge Circular Road, 19 Calcutta, India |
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Abstract: | Summary Different staining procedures, various digestion methods and autoradiographic techniques were employed to study the structure and composition of the nucleolus and of the nucleolonema, after unmasking the latter by adenosine treatment. The presence of DNA, RNA, protein and lipid in these structures has been shown. It has been demonstrated that the filamentous structure within the nucleolus — the nucleolonema— has a core of DNA, around which RNA and protein have accumulated. The structure of the nucleolonema suggests that it is in a highly active state, in synthesizing ribosomal RNA and protein.We take the opportunity to express our gratefulness to the Director, Prof. Dr. Hans Lettré, for providing facilities to work in this Institute. We like to thank our other colleagues, particularly Dr. N. Paweletz, for their valuable help during the course of the investigations. |
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Keywords: | Nucleolus Filamentous Structures Composition |
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