Development of a pair of bifunctional expression vectors for <Emphasis Type="Italic">Escherichia coli</Emphasis> and <Emphasis Type="Italic">Bacillus licheniformis</Emphasis> |
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Authors: | Dandan Niu Zheng-Xiang Wang |
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Institution: | (1) The Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Southern Yangtze University, Wuxi, 214036, China |
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Abstract: | A pair of bifunctional expression vectors, pBL-WZX and pHY-WZX, for Escherichia coli and Bacillus licheniformis was constructed to express interesting genes in a secretory manner. The vectors contain an expression cassette consisted
of the promoter and signal peptide region of B. licheniformis amyL as well as an artificial multiple cloning site and a terminator and utilize kanamycin-resistance and/or tetracycline-resistance
for selection in both B. licheniformis and E. coli. Both vectors contain a part of 3′ terminal fragment of B. licheniformis amyL. The 5′-terminal or 3′-terminal fragment of B. licheniformis amyL can cause the integration and amplification of expression cassette in the chromosome of B. licheniformis under a kanamycin-selection pressure. pBL-WZX is an integrational vector while pHY-WZX is free one for B. licheniformis. Both vectors were succeeded in secretory expression of manL in both B. licheniformis and E. coli. |
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Keywords: | Expression vector Bacillus licheniformis Promoter of amyL |
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