Internode length in Pisum. Estimation of GA1 levels in genotypes Le, le and led |
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Authors: | John J Ross James B Reid Paul Gaskin Jake MacMillan |
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Institution: | Dept. of Botany, Univ. of Tasmania, Box 252C, GPO, Hobart, Tasmania, 7001, Australia;AFRC Research Group, School of Chemistry, Univ. of Bristol, Bristol, U.K. |
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Abstract: | The levels of GA1, 3-epiGA1 and GA8 in genotypes Le, le and led of Pisum sativum L. were determined by gas chromatography-selected ion monitoring (GC-SIM) after feeds of 3H, 13C]-GA20 to each genotype. The levels of endogenous and 13C]-labelled metabolites were determined by reverse isotope dilution with unlabelled GA1, 3-epiGA1 and GA8. The results demonstrate a quantitative relationship between the level of GA1 and the extent of elongation both on a per plant and a per g fresh weight basis. These results are consistent with previous findings in peas and other species possessing a predominant early 13-hydroxylation pathway for GA biosynthesis. The levels of 3-epiGA1 also decreased in the genotypic sequence Le, le, led although not as rapidly as for the level of GA1. This may suggest that the alleles at the le locus also influence the formation of 3-epiGA1. |
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Keywords: | Dwarf elongation gibberellin content gibberellin metabolism internode length Pisum sativum |
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