Structural Studies of Heterochiral DNA/DNA,RNA/RNA,AND DNA/RNA Duplexes |
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Authors: | Hidehito Urata Hana Shimizu Masao Akagi |
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Institution: | Osaka University of Pharmaceutical Sciences , Takatsuki , Osaka , Japan |
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Abstract: | Using DNA and RNA heptanucleotides containing an unnatural L-nucleotides as well as the complementary strands, effects of the introduction of an L-nucleotide on the structure of DNA/DNA, RNA/RNA, and DNA/RNA duplexes were investigated by circular dichroism experiments and RNase H-mediated RNA strand cleavage reaction. The results suggested that the substitution of the central D-nucleotide with an L-nucleotide in the duplexes causes the significant structural alterations as the duplex structures change to conformations with more B-form similarities. |
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Keywords: | DNA RNA DNA/RNA hybrid duplex Duplex structure Homochiral Heterochiral |
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