Synthesis and Application of Negatively Charged PNA Analogues |
| |
| Authors: | Vladimir A Efimov Oksana G Chakhmakhcheva Eric Wickstrom |
| |
| Institution: | 1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry , Moscow, Russia;2. Department of Biochemistry and Molecular Pharmacology and Kimmel Cancer Center , Thomas Jefferson University , Philadelphia, Pennsylvania, USA |
| |
| Abstract: | Negatively charged DNA mimics containing phosphonate analogues of peptide nucleic acids were designed, and their physicochemical and biological properties were evaluated in the comparison with natural oligonucleotides, classical peptide nucleic acids, and morpholino phosphorodiamidate oligonucleotide analogues. The results obtained revealed a high potential of phosphonate-containing PNA derivatives for a number of biological applications, such as diagnostic, nucleic acids analysis, and inhibition of gene expression. |
| |
| Keywords: | DNA analogues Phosphono-PNA analogues Hybridization Inhibition Diagnostic Antisense reagents |
|
|
