Denaturing Reversed-Phase HPLC Using a Mobile Phase Containing Urea for Oligodeoxynucleotide Analysis |
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| Authors: | Suntara Fueangfung Yinan Yuan |
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| Institution: | 1. Department of Chemistry, Michigan Technological University, Houghton, Michigan, USA;2. School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, Michigan, USA |
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| Abstract: | Denaturing reversed-phase (RP) high performance liquid chromatography (HPLC) is usually achieved by elevating column temperature. In this article, an alternative method involving using a mobile phase that contains urea and performing HPLC at room temperature is described. The efficacy of the new method was demonstrated by analyzing a 61-mer oligodeoxynucleotide (ODN) and double-stranded (ds) ODNs. The multiple peaks of the 61-mer ODN under normal conditions merged into one under the denaturing conditions. The broad single peaks of dsODNs under normal conditions were split into two sharp peaks. |
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| Keywords: | Denaturing HPLC urea oligodeoxynucleotide DNA |
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