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Hydroxyl-Functionalized DNAs with Different Linkers and Their Complmentary Duplex Stability |
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| Authors: | Di Zhang Yamin Li Liang Xu Maosheng Cheng Ying Zhou Junlin He |
| Affiliation: | 1. School of Pharmaceutical Engineering , Shenyang Pharmaceutical University , Shenyang, P. R. China;2. Beijing Institute of Pharmacology and Toxicology , Beijing, P. R. China;3. Beijing Institute of Pharmacology and Toxicology , Beijing, P. R. China;4. College of Life Science , Guizhou University , Guizhou, P. R. China;5. Beijing Institute of Pharmacology and Toxicology , Beijing, P. R. China;6. School of Pharmaceutical Engineering , Shenyang Pharmaceutical University , Shenyang, P. R. China;7. College of Life Science , Guizhou University , Guizhou, P. R. China |
| Abstract: | Tert-butyldiphenylsilyl (TBDPS) was testified to be an appropriate orthogonal protecting group for novel 7-hydroxyl-functionalized 8-aza-7-deaza-2′-deoxyadenosine analogues. It was stable in partial and complete hydrogenation reactions used for the different linker preparation. The corresponding phosphoramidites and hydroxyl-functionalized oligodeoxynucleotides were synthesized and identified. The thermal effect of the hydroxyl group with different linkers on DNA duplexes was evaluated. It provided a feasible strategy for the preparation of hydroxyl-functionalized DNAs for the nucleic acid research. |
| Keywords: | Tert-butyldiphenylsilyl 8-aza-7-deazaza-2′-deoxyadenosine hydroxypropargyl hydroxypropenyl oligodeoxynucleotides thermal stability |