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CRISPR interference as a titratable,trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002
Institution:1. Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, United States;2. Microbiology Doctoral Training Program, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI 53706, United States;3. Department of Environmental Chemistry and Technology, University of Wisconsin-Madison, 660 N Park St, Madison, WI 53706, United States;4. Departmentof Chemistry and Biochemistry, University of Colorado-Boulder, 596 UCB, Boulder, CO 80309, United States;5. Renewable and Sustainable Energy Institute, University of Colorado-Boulder, 027 UCB, Boulder, CO 80309, United States;1. Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA;2. Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA;1. Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, India;2. DBT-Pan IIT Center for Bioenergy, Indian Institute of Technology Bombay, Mumbai 400076, India;3. Wadhwani Research Center for Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India;4. Reliance Technology Group, Reliance Industries Limited, Reliance Corporate Park, Ghansoli, Navi Mumbai 400701, India;1. Department of Chemical and Biological Engineering, University of Wisconsin – Madison, 1415 Engineering Dr., Madison, WI 53706, United States;2. Department of Environmental Chemistry and Technology, University of Wisconsin – Madison, 660 N Park St., Madison, WI 53706, United States;1. Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan;2. Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 70101, Taiwan;1. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China;2. Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China;3. Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China;4. Dalian National Laboratory for Clean Energy, Dalian, China;5. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China;1. Institute for Genomics and Proteomics University of California, Los Angeles, CA 90095, USA;2. Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, USA
Abstract:
Keywords:CRISPRi  Tunable  Cyanobacteria  Lactate  Chemical production  Carboxysome  Phycobilisome  Synthetic biology
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