| 氨基酸产生菌E_(65)中质粒的分离与鉴定 |
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| 引用本文： | 张明,陈慎,申同健.氨基酸产生菌E_(65)中质粒的分离与鉴定[J].中国生物化学与分子生物学报,1988,4(4):338-344. |
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| 作者姓名： | 张明 陈慎 申同健 |
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| 作者单位： | 中国科学院生物物理研究所 北京
(张明，陈慎)，中国科学院生物物理研究所 北京(申同健) |
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| 摘 要： | 在棒杆菌与短杆菌中,用微量质粒分离方法筛选到一株含多种质粒的乙酰短杆菌(Brevibacterium actylicum)E_(65)菌株,该菌株具有产氨基酸特性。进一步大量制备其质粒DNA,并进行氧化绝密度梯度超离心纯化,以及电泳分析,电镜观察,并对质粒进行分离与鉴定,从而确定了每种质粒的分子量大小。
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| 关 键 词： | 氨基酸产生菌 质粒分离与鉴定 质粒电镜分析 |
| 收稿时间： | 1988-08-20 |
ISOLATION AND CHARACTERIZATION OF PLASMIDS. IN AN AMINO ACID PRODUCING BACTERIUM E65 |
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| Zhang,Ming Chen,Shen Shen,Tong-Jian.ISOLATION AND CHARACTERIZATION OF PLASMIDS. IN AN AMINO ACID PRODUCING BACTERIUM E65[J].Chinese Journal of Biochemistry and Molecular Biology,1988,4(4):338-344. |
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| Authors: | Zhang Ming Chen Shen Shen Tong-Jian |
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| Institution: | (Institute of Biophysics, Academia Sinica, Beijing |
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| Abstract: | From a number,of corynebacteria and brevibacteria strains, three plasmids are found in an amino acid producing strain E65. These plasmids are isolated on large scale, purified by centrifugation to equilibrium in cesium chloride-ethidium bromide density gradients, and are characterized with agarose gel electrophoresis and eletron microscopy. The molecular weight and size of these plasmids is determined. |
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| Keywords: | Amino acid producing strain Isolation and preparation of plasmid DNA Electron microscopy of plasmid |
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