| Abstract: | The aim of the investigations was to obtain — from the parent strain Aspergillus niger A.n. 33 — selectants with an increased ability of cellulolytic enzymes biosynthesis. Own selection methods allowed to receive two selectants A.n. 33/2 and A.n. 33/20 characterized by enhanced activities of saccharifying cellulase (respectively 0.11 and 0.14 FPU/cm3), endo-beta-1,4-glucanase (15.4 and 21.8 U/cm3) and cellobiase (0.6 and 1.4 IU/cm3) as compared with the parent strain (FPA — 0.09 IU, CMC — 8.2 U and CB — 0.1 IU/cm3). Moreover, the selectants differed in shape and size of conidial heads, in shape and colour of conidia, as well as in structure and shape of hyphae. Enzyme preparations obtained after ultrafiltration of liquid cultures were characterized by following activites: FPA-4–16 IU, CMC — 900–1800 U, CB — 60–120 IU and xylanase — 250–280 IU/cm3. |
