Expression of major antigen domains of E2 gene of CSFV and analysis of its immunological activity |
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Authors: | Hong Tian Xiang-tao Liu Jing-yan Wu You-jun Shang Tao Jiang Hai-xue Zheng Qing-ge Xie |
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Institution: | (1) Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China |
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Abstract: | E2 is an envelope glycoprotein of Classical swine fever virus (CSFV) and contains sequential neutralizing epitopes to induce
virus-neutralizing antibodies and mount protective immunity in the natural host. In this study, four antigen domains (ABCD)
of the E2 gene was cloned from CSFV Shimen strain into the retroviral vector pBABE puro and expressed in eukaryotic cell (PK15)
by an retroviral gene expression system, and the activity of recombinant E2 protein to induce immune responses was evaluated
in rabbits. The results indicated that recombinant E2 protein can be recognized by fluorescence antibodies of CSFV and CSFV
positive serum (Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China) using Western
blot, indirect immunofluorescence antibody test (IFAT) and ELISA, Furthermore, anti-CSFV specific antibodies and lymphocyte
proliferation were elicited and increased by recombinant protein after vaccination. In the challenge test, all of rabbits
vaccinated with recombinant protein and Chinese vaccine strain (C-strain) were fully protected from a rabbit spleen virus
challenge. These results indicated that a retroviral-based epitope-vaccine carrying the major antigen domains of E2 is able
to induce high level of epitope-specific antibodies and exhibits similar protective capability with that induced by the C-strain,
and encourages further work towards the development of a vaccine against CSFV infection.
Foundation items: The National “973” (2005CB523201); Key Technology R&D Programme (2006BAD06A03). |
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Keywords: | Classical swine fever virus (CSFV) E2 gene Antigen domains Retroviral vector Immunological activity |
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