Comparative Efficacy of Abscisic Acid and Methyl Jasmonate for Indirect Somatic Embryogenesis in Medicago sativa L. |
| |
Authors: | Izabela Rudu? Ewa K?pczyńska Jan K?pczyński |
| |
Institution: | (1) Department of Plant Physiology and Biotechnology, Szczecin University, Wąska 13, 71-415, Szczecin, Poland |
| |
Abstract: | The effects of methyl jasmonate (MeJA) in relation to abscisic acid (ABA) on different phases of somatic embryogenesis were
studied in Medicago sativa L. Different concentrations of both the growth inhibitors (0.0, 0.5, 5.0, 50.0 and 500.0 μM) were tested in five distinct phases of somatic embryogenesis, viz., induction, proliferation, differentiation, maturation
and regeneration. Like ABA, MeJA also inhibited callus induction, callus growth, proliferation of embryogenic suspension as
well as germination and conversion of somatic embryos. However, its inhibitory effects on various phases of somatic embryogenesis
were less pronounced as compared to that due to ABA. In contrast to ABA, MeJA did not have any significant influence on the
development of somatic embryos when applied in the differentiation phase. The study showed that ABA used routinely as an inducer
of somatic embryo maturation in M. sativa could not be replaced by MeJA. |
| |
Keywords: | Abscisic acid Alfalfa Jasmonates Medicago sativa L Methyl jasmonate Somatic embryogenesis |
本文献已被 SpringerLink 等数据库收录! |
|