Antipathogenic potential of marine <Emphasis Type="Italic">Bacillus</Emphasis> sp. SS4 on <Emphasis Type="Italic">N</Emphasis>-acyl-homoserine-lactone-mediated virulence factors production in <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> (PAO1) |
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Authors: | K Syed Musthafa V Saroja S Karutha Pandian A Veera Ravi |
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Institution: | (1) Department of Biotechnology, Alagappa University, Karaikudi, 630 003, India; |
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Abstract: | Antipathogenic therapy is an outcome of the quorum-sensing inhibition (QSI) mechanism, which targets autoinducer-dependent
virulent gene expression in bacterial pathogens. N-acyl homoserine lactone (AHL) acts as a key regulator in the production of virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1 and violacein pigment production in Chromobacterium violaceum. In the present study, the marine bacterial strain SS4 showed potential QSI activity in a concentration-dependent manner
(0.5–2 mg/ml) against the AHL-mediated violacein production in C. violaceum (33–86%) and biofilm formation (33–88%), total protease (20–65%), LasA protease (59–68%), LasB elastase (36–68%), pyocyanin
(17–86%) and pyoverdin productions in PAO1. The light and confocal laser scanning microscopic analyses confirmed the reduction
of the biofilm-forming ability of PAO1 when treated with SS4 extract. Furthermore, the antibiofilm potential was confirmed
through static biofilm ring assay, in which ethyl acetate extract of SS4 showed concentration-dependent reduction in the biofilm-forming
ability of PAO1. Thus, the result of this study clearly reveals the antipathogenic and antibiofilm properties of the bacterial
isolate SS4. Through 16S rDNA analysis, the strain SS4 was identified as Bacillus sp. (GenBank Accession Number: GU471751). |
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