Identification and characterization of a novel endoglucanase (CMCase) isolated from the larval gut of Bombyx mori |
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| Institution: | 1. Jiangsu University of Science and Technology, Zhenjiang 212018, China;2. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China;1. Laboratory of Silkworm Science, Institute of Biological Control, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Hakozaki 6-10-1, Fukuoka 812-8581, Japan;2. Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Hakozaki 6-10-1, Fukuoka 812-8581, Japan |
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| Abstract: | While screening for cellulase-producing fungi from insect gut, a fungus with high endoglucanase (carboxymethyl cellulase; CMCase) activity was isolated from the larval gut of Bombyx mori. Based on morphological characteristics and using an 18S rRNA-based molecular phylogenetic approach, the fungus, strain BMC-2, was identified as a Mucor sp. expressing a novel alkalotolerant cellulase. The maximum production of cellulase by the BMC-2 strain was observed at 55 °C and pH 8.0. The CMCase activity was inhibited by Cu2 + > Na+ > Zn2 + > Mg2 + > Ba2 +, and induced by Ca2 +, Mn2 +, Fe2 +, and K+. |
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