Radioprotective effects of Dragon's blood and its extract against gamma irradiation in mouse bone marrow cells |
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| Institution: | 1. School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China;2. China Institute of Atomic Energy, Beijing 102413, PR China;3. Lanzhou Institute of Modern Physics, Lanzhou 730000, PR China;1. Crustacean Molecular Biology and Genomics Lab, Department of Animal Health and Management, Alagappa University, Science Block 4th Floor, Burma Colony, Karaikudi, 630004, Tamil Nadu, India;2. Mandapam Regional Centre, Central Marine Fisheries Research Institute, Mandapam, Tamil Nadu, India;1. School of Health and Biomedical Sciences, RMIT University, Victoria, 3083, Australia;2. School of Chemistry, The University of Melbourne, Victoria, 3010, Australia;3. Alfred Hospital Radiation Oncology, Melbourne, Australia;4. Dept. of Clinical Radiology, Hiroshima Int. University, Hiroshima, 739-452, Japan;1. School of Chemistry, Monash University, Clayton, Victoria 3800, Australia;2. Chemistry Department, Mulawarman University, Samarinda 75123, Indonesia;1. School of Life Science, Beijing Institute of Technology, Beijing, China;2. Key Lab of Industrial Fermentation Microbiology, Ministry of Education, College of Bioengineering, Tianjin University of Science and Technology, China |
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| Abstract: | PurposeThe radioprotective effects of Dragon's blood (DB) and its extracts (DBE) were investigated using the chromosomal aberrant test, micronucleus and oxidative stress assay for anti-clastogenic and anti-oxidative activity.Materials and methodsAdult BALB/C mice were exposed to the whole body irradiation with 4 Gy 60Co γ-rays. DB and DBE were administered orally once a day from 5 days prior to irradiation treatment to 1 day after irradiation. The mice were sacrificed on 24 h after irradiation. The cells of bone marrow were measured by counting different types of chromosomal aberrations and the frequency of micronuclei. Oxidative stress response was carried out by analysis of serum from blood.ResultsDB and DBE significantly decreased the number of bone marrow cells with chromosome aberrations after irradiation with respect to irradiated alone group. The administration of DB and DBE also significantly reduced the frequencies of micronucleated polychromatic erythrocytes (MPCE) and micronucleated normochromatic erythrocytes (MNCE). In addition, DB and DBE markedly increased the activity of antioxidant enzymes and the level of antioxidant molecular. Malondialdehyde (MDA) and nitric oxide (NO) levels in serum were significantly reduced by DB and DBE treatment.ConclusionsOur data suggested that DB and DBE have potential radioprotective properties in mouse bone marrow after 60Co γ-ray exposure, which support their candidature as a potential radioprotective agent. |
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| Keywords: | Dragon's blood Radioprotective effects Irradiation Bone marrow Oxidative stress |
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