Simple and rapid screening of entomopathogenic fungi having radical-scavenging and anticancer activities |
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| Institution: | 1. State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, People''s Republic of China;2. Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Zhejiang University, Hangzhou, People''s Republic of China;3. Institute of Insect Sciences, Zhejiang University, Hangzhou, People''s Republic of China;4. EH Graham Centre for Agricultural Innovation, Charles Sturt University, Orange, NSW, Australia;1. Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China;2. Department of Animal Science, Fujian Vocational College of Agriculture, Fuzhou, Fujian 350007, China;3. Biology Centre of the Academy of Sciences of the Czech Republic, Institute of Entomology, Brani?ovská 31, 37005 ?eské Budějovice, Czech Republic;1. Holoce Ecosystem Conservation Research Institute (HECRI), Gapchon-myon, Dunae-myon and Wuchon-myon, Hweongsung-gun, Gangwon-do, Republic of Korea;2. Crop Protection Division, Department of Crop Life Safety, National Academy of Agricultural Science, 126 Suin-ro, Kweonseon-gu, Suwon, Gyounggi-do 441-707, Republic of Korea |
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| Abstract: | Entomopathogenic fungal metabolites exhibit a wide variety of insecticidal, antimicrobial, anticancer, antioxidant, and antiviral activities, and they have been suggested as potential candidates for the development of new bioactive agents. The rapid and simple screening of entomopathogenic fungi with such bioactivities is important for investigating bioactive substances. The radical-scavenging and anticancer activities of 28 species and 20 genera of 342 isolates of entomopathogenic fungi were evaluated using only culture filtrates. As a result, 23 isolates of Metarhizium anisopliae var. anisopliae, 1 isolate of Pochonia sp., and 1 isolate of Aspergillus sp. showed high radical-scavenging activity. Additionally, anticancer activity in HCT116 human colon cancer cells was also confirmed for selected culture filtrates. This simple screening method of entomopathogenic fungi using culture filtrates may be useful for the rapid detection of related substances with pharmacological activity from a large number of fungal samples. |
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