丹参抗性基因SmORA1的克隆及诱导表达分析

该研究采用PCR方法从丹参中克隆出一条乙烯应答因子结合蛋白(ERF)转录因子编码基因,命名为SmORA1,GenBank登录号为KT359598。经分析发现该基因全长648bp,不包含内含子,编码206个氨基酸残基。编码蛋白SmORA1含有典型的AP2结合结构域。表达分析结果表明,SmORA1主要在丹参根中表达,且该基因的表达明显受到茉莉酸甲酯(MeJA)、脱落酸(ABA)、乙烯(ET)、机械创伤和病原菌等逆境信号的诱导,但低温和脱水情况下SmORA1表达下调。研究表明,SmORA1参与丹参生物胁迫反应,可整合JA、ABA、ET和病原菌等胁迫信号途径。

Cloning and Induced Expression of SmORA1 Gene from Salvia miltiorrhiza

In this study,one gene encoding ethylene responsive factor binding protein (ERF) was cloned from Salvia miltiorrhiza by RT-PCR,which was named as SmORA1(GenBank accession number:KT359598).SmORA1 DNA sequence consists of 648 bp without introns,and encoding 206 amino acid residues.Bioinformatics analysis showed that SmORA1 contain typical AP2 binding domain of ERF family.The real-time quantitative PCR analysis revealed that SmORA1 was mainly expressed in the roots,furthermore it can be induced by methyl jasmonate (MeJA),abscisic acid (ABA),ethylene (ET),physical wounding and pathogens.While the expression level of SmORA1 was down-regulated under low temperature or dehydration treatment.These results indicated that SmORA1 was involved in plant stress responses,and integrated JA,ET,ABA and pathogen signals in S.miltiorrhiza.