Characterization of recombinant soluble carcinoembryonic antigen cell adhesion molecule 1 |
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Authors: | Schumann Detlef Huang Jie Clarke Patrick E Kirshner Julia Tsai Shih-wa Schumaker Verne N Shively John E |
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Institution: | Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA. |
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Abstract: | Carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) is a type 1 transmembrane, homotypic cell adhesion protein expressed on epithelial and hematopoietic cells. CEACAM1 has four major isoforms with three or four immunoglobulin (Ig)-like ectodomains and either long or short cytoplasmic domains. In a 3D model of breast epithelial cell morphogenesis, CEACAM1 plays an essential role in lumen formation J. Cell Sci. 112 (1999) 4193]. Two soluble ectodomain isoforms of CEACAM1 expressed in myeloma cells were immunologically active and highly glycosylated. The molecular weights of the 3-ecto- and 4-ectodomain isoforms were 90 and 110kDa, respectively, and monomers by sedimentation equilibrium centrifugation. Both isoforms were prolate ellipsoids with axial ratios of 6 for the 3-ecto- and 8 for 4-ectodomain isoforms, respectively, by size exclusion chromatography and analytical ultracentrifugation. Both isoforms caused a significant reduction in lumen formation when tested in the 3D model culture system. |
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Keywords: | CECAM1 Cell morphogenesis Immunoglobulin domains Sedimentation equilibrium Analytical ultracentrifugation |
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