300 MHz PMR studies on the conformation of the hexanucleotide, 2'OMeGpApApUpApPsi, from the anticodon loop of torula yeast tRNAphe

A complete temperature study of the proton resonances of the hexanucleotide, 2′-OMeGpApApYpApψ, from Torula yeast tRNA^phe has been carried out at 300 MHz. The data has been interpreted in terms of a base stacked oligomer in which the glycosyl conformation of the Y-nucleoside changes from syn to anti with temperature increase. An alternative structure for the Y-base is proposed to permit this conformational change.