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中国角叉菜属(Chondrus)的分类研究:形态观察和rbcL序列分析
引用本文:苏乔,栾日孝,安利佳,祖元刚. 中国角叉菜属(Chondrus)的分类研究:形态观察和rbcL序列分析[J]. 植物研究, 2002, 22(1): 10-18
作者姓名:苏乔  栾日孝  安利佳  祖元刚
作者单位:1. 大连理工大学生物工程系,大连,116012
2. 东北林业大学,哈尔滨,150040
摘    要:
采用rbcL序列分析结合传统形态观察的方法 ,对我国福建省平潭县和漳浦县、山东省青岛市及辽宁省大连市采集的角叉菜属样品进行了研究。新鲜或硅胶干燥样品被用于总DNA提取并用于PCR反应 ,PCR扩增产物回收后直接用于测序反应 ,序列数据经ClustalX1 .8软件排序后 ,采用MEGA2 .1软件根据Kimura’s双因子法计算遗传距离 ,并应用邻接法 (neighbor - joint ing)和最大简约法 (MaximumParsimony)构建系统树 ,Bootstrap法 (5 0 0次重复 )评估分支可信度。研究表明 :确认中国存在Chondrusocellatus、Chondrusnipponicus和Chondrusarmatus三种角叉菜 ;原报道产于我国东南沿海的角叉菜Chondrusocellatus应为Grateloupiaimbricata ;原报道产于大连小平岛的角叉菜Chondrusyendoi应为Mazzaellajaponica。

关 键 词:角叉菜属  分类  rbcL
修稿时间:2001-10-22

TAXONOMIC STUDIES OF CHONDRUS,CHINA ON THE BASIS OF MORPHOLOGY AND rbcL SEQUENCES
SU Qiao LUAN Ri xiao AN Li jia ZU Yuan gang. TAXONOMIC STUDIES OF CHONDRUS,CHINA ON THE BASIS OF MORPHOLOGY AND rbcL SEQUENCES[J]. Bulletin of Botanical Research, 2002, 22(1): 10-18
Authors:SU Qiao LUAN Ri xiao AN Li jia ZU Yuan gang
Affiliation:SU Qiao 1 LUAN Ri xiao 1 AN Li jia 1 ZU Yuan gang 2
Abstract:
Morphological observations and rbcL sequence analyses of chondrus collected from Pingtan and Zhangpu of Fujian province, Qingdao of Shandong province and Dalian of Liaoning province were made. Total DNA were extracted from fresh or silica gel-desiccated plants and used as templates for polymerase chain reactions. Purified polymerase chain reaction products were directly sequenced. The rbcL sequences were aligned using Clustal X1.8 computer program, then used to calculate distance matrix with Kimura's two-parameter method and construct phylogenetic trees by applying neighbor-joining and Maximum Parsimony analyses using MEGA2.1 computer program. Bootstrap analyses were carried out to evaluate statistical reliability based on 500 resamplings of the data set. The results indicated three Chondrus species in China: Chondrus ocellatus , Chondrus nipponicus and Chondrus armatus . The results also suggested that Chondrus ocellatus which was described from specimen collected at Southeast seashore of China should be Grateloupia imbricata and Chondrus yendoi which was described from specimen collected from Xiao Pingdao, Dalian should be Mazzaella japonica .
Keywords:Chondrus  taxonomy  rbcL
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