Effects of light, magnesium and sucrose on leaf anatomy, photosynthesis, starch and total sugar accumulation, in kiwifruit culturedin vitro |
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Authors: | Kortessa Dimassi-Theriou Artemios M Bosabalidis |
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Institution: | (1) Department of Horticulture, Faculty of Agriculture, Aristotle University, 54006 Thessaloniki, Greece;(2) Department of Botany, School of Biology, Aristotle University, 54006 Thessaloniki, Greece |
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Abstract: | Shootlets of kiwifruit plants (Actinidia deliciosa) were culturedin vitro. Combinations of light intensity, Mg and sucrose in the cultures showed that an increase of light intensity resulted in a
corresponding increase of the relative size of the leaf mesophyll cells and in a decrease of the numbers of chloroplasts and
contained starch grains. The addition of sucrose to the substrate media negatively affected the size of the mesophyll cells
under normal Mg concentration (35 mg l−1), and positively under high Mg concentration (105 mg l−1 ). Sucrose further resulted in an increase in the numbers of chloroplasts and contained starch grains. The photosynthetic
capacity of leaves greatly increased when Mg concentration was enhanced and sucrose was excluded from the nutrient substrate.
Total sugar accumulation in all treatments was favoured by normal light intensity and addition of sucrose. |
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Keywords: | Actinidia deliciosa in vitro culture |
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