Foam behavior of biological media |
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Authors: | H Buchholz K Kalischewski K Schügerl |
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Institution: | (1) Institut für Technische Chemie der Universität Hannover, Callinstr. 3, D-3000 Hannover 1, Federal Republic of Germany |
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Abstract: | Summary The surface tension and foaminess of (a) unlimited, (b) substrate limited, and (c) oxygen transfer limited growth media of Hansenula polymorpha were measured using methanol, ethanol or glucose as a substrate.The time dependence of can be described by the Avrami-Überreiter relationship: log (2.3 log V)=n log t+log b, where V = (O – eq/(t – eq, and O, t and eq are at tM=0, tM=t and tM (equilibrium value).The constants n and b are functions of the fermentation time tF as long as the growth is unlimited but they are constant in the state of limited growth. With glucose substrate, the foaminess can be presented as a definite function of the time, tDG, which is necessary to attain eq. With alcohol as a substrate no definite (tDG) function was found.Symbols b
constant in Eq. (1)
- n
constant in Eq. (1)
- S
substrate concentration
- T
temperature
- tM
time h (measured from the beginning of the determination of the surface tension )
- tF
cultivation time h (measured from the time of inoculation)
- tDG
time (min) necessary to attain the equilibrium surface tension )
- X
dry biomass concentration (gl–1)
- V
(O – eq)/(t – eq)
- VS
equilibrium volume of the foam (cm3)
- VG
volumetric gas flow rate during the estimation of (cm3 s–1)
- vvm
volumetric gas flow rate with regard to the volume of the medium (min–1)
- wSG
superficial gas velocity (cm s–1)
- m
maximum specific growth rate (h–1)
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VS/VG foaminess (s)
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surface tension, mMm–1 (milli Newton m–1)
- O
at tM=0
- eq
equilibrium surface tension ( at tM)
- t
at tM=t
- HP
probes from Hansenula polymorpha cultivation
- NLG
non limited growth
- OTLG
oxygen transfer limited growth
- SLG
substrate limited growth |
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Keywords: | |
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