C1n3缺失对酿酒酵母生长的影响

Cln3是酿酒酵母G1期周期蛋白中的一种，为了研究Cln3在细胞周期与形态发生中的作用，我们构建了酿酒酵母CLN3基因的缺失株，并对其表型进行了分析。结果显示，cln3缺失株对α信息素的敏感性增强，α信息素诱导的细胞周期停滞现象明显大于野生型菌株，这种增强作用不受Sgvl因子的影响。同时，与野生菌相比cln3缺失株的细胞形态也有明显变化，双倍体cln3缺失株细胞的顶端生长能力增强而单倍体细胞的侵入生长能力则受到抑制。结果表明，与酿酒酵母的另外两个G1期周期蛋白Clnl、Cln2不同，Cln3在形态发生中有其独特的功能与作用方式。

The role of Cln3 in filamentous growth and invasive growth of Saccharomyces cerevisiae]

Cln3 is one of G1 cyclins in Saccharomyces cerevisiae. In order to study the function of Cln3 in cell cycle and morphogenesis, we constructed a cln3 null mutant and analyzed its phenotype. Our results indicated that the cln3 null mutant was more sensitive to alpha pheromone, and arrested at G1 phase. The hypersensitivity to alpha-pheromone was not suppressed by overexpression of SGV1. The null mutant showed a different phenotype with that of the other two G1 cyclin mutants. The filamentous growth in diploid cells of cln3 mutant was stronger than that in wild type cells, while invasive growth of the haploid cells was partially inhibited. The results suggested that the Cln3 plays a unique function in morphogenesis under a different mechanism with that used by Cln1 and Cln2.