Expression of Cholinergic,Insulin, Vitamin D Receptors and GLUT 3 in the Brainstem of Streptozotocin Induced Diabetic Rats: Effect of Treatment with Vitamin D3 |
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Authors: | Peeyush Kumar T Paul Jes Antony Sherin Paulose C S |
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Institution: | (1) Cell Biology-Cardio Vascular Unit, Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, P.O. BOX 3354, Riyadh, 11211, Saudi Arabia;(2) Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Cochin University of Science and Technology, Cochin, 682 022, Kerala, India; |
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Abstract: | Complications arising from diabetes mellitus include cognitive deficits, neurophysiological and structural changes in the
brain. The current study investigated the expression of cholinergic, insulin, Vitamin D receptor and GLUT 3 in the brainstem
of streptozotocin-induced diabetic rats. Radioreceptor binding assays and gene expression were done in the brainstem of male
Wistar rats. Our results showed that Bmax of total muscarinic, muscarinic M3 receptors was increased and muscarinic M1 receptor was decreased in diabetic rats compared
to control. A significant increase in gene expression of muscarinic M3, α7 nicotinic acetylcholine, insulin, Vitamin D3 receptors, acetylcholine esterase, choline acetyl transferase and GLUT 3 were observed in the brainstem of diabetic rats.
Immunohistochemistry studies of muscarinic M1, M3 and α7 nicotinic acetylcholine receptors confirmed the gene expression at
protein level. Vitamin D3 and insulin treatment reversed diabetes-induced alterations to near control. This study provides an evidence that diabetes
can alter the expression of cholinergic, insulin, Vitamin D receptors and GLUT 3 in brainstem. We found that Vitamin D3 treatment could modulate the Vitamin D receptors and plays a pivotal role in maintaining the glucose transport and expressional
level of cholinergic receptors in the brainstem of diabetic rats. Thus, our results suggest a therapeutic role of Vitamin
D3 in managing neurological disorders associated with diabetes. |
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