Diversity and distribution in hypersaline microbial mats of bacteria related to Chloroflexus spp |
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Authors: | Nübel U Bateson M M Madigan M T Kühl M Ward D M |
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Institution: | Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, Montana, USA. unuebel@dsmz.de |
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Abstract: | Filamentous bacteria containing bacteriochlorophylls c and a were enriched from hypersaline microbial mats. Based on phylogenetic analyses of 16S rRNA gene sequences, these organisms form a previously undescribed lineage distantly related to Chloroflexus spp. We developed and tested a set of PCR primers for the specific amplification of 16S rRNA genes from filamentous phototrophic bacteria within the kingdom of "green nonsulfur bacteria." PCR products recovered from microbial mats in a saltern in Guerrero Negro, Mexico, were subjected to cloning or denaturing gradient gel electrophoresis and then sequenced. We found evidence of a high diversity of bacteria related to Chloroflexus which exhibit different distributions along a gradient of salinity from 5.5 to 16%. |
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