A DNA marker closely linked to the <Emphasis Type="Italic">vrs1</Emphasis> locus (row-type gene) indicates multiple origins of six-rowed cultivated barley (<Emphasis Type="Italic">Hordeum vulgare</Emphasis> L.) |
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| Authors: | K Tanno S Taketa K Takeda T Komatsuda |
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| Institution: | (1) National Institute of Agrobiological Resources, Kannondai, Tsukuba 305-8602, Japan, JP;(2) Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, 305-8572, Japan Present address: K. Tanno, Research Institute for Bioresources, Okayama University, Chuo, Kurashiki 710-0046, Japan, e-mail: tannok@rib.okayama-u.ac.jp Tel.: +81-86-424-1661, Fax: +81-86-434-1249), JP;(3) Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan, JP;(4) Research Institute for Bioresources, Okayama University, Chuo, Kurashiki 710-0046, Japan, JP |
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| Abstract: | The origin of six-rowed cultivated barley was studied using a DNA marker cMWG699 closely linked to the vrs1 locus. Restriction patterns of the PCR-amplified product of the cMWG699 locus were examined in 280 cultivated (Hordeum vulgare ssp. vulgare) and 183 wild (H. vulgare ssp. spontaneum) barleys. Nucleotide sequences of the PCR products were also examined in selected accessions. Six-rowed cultivated barleys
were divided into two distinct groups, types I and II. Type I six-rowed cultivated barley was distributed widely while type
II six-rowed cultivated barley was found only in the Mediterranean region. The type I sequence was also found in a wild barley
accession from Turkmenistan whereas the type II sequence was also found in a two-rowed cultivated barley from North Africa
and a wild barley from Morocco. These results suggested that the six-rowed type I and II barleys were derived from two-rowed
type I and II barleys, respectively, by independent mutations at the vrs1 locus.
Received: 3 November 2000 / Accepted: 17 April 2001 |
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| Keywords: | Barley Six-rowed Origin The vrs1 locus Nucleotide sequence |
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