Development of a simple particle bombardment device for gene transfer into plant cells |
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| Authors: | Daniel C. W. Brown Lining Tian Del J. Buckley Mark Lefebvre Andy McGrath John Webb |
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| Affiliation: | (1) Plant Research Centre, Agriculture Canada, Central Experimental Farm, Ottawa, Canada;(2) Centre for Food and Animal Research, Agriculture Canada, Central Experimental Farm, Ottawa, Canada;(3) Biology Department, Carleton University, Ottawa, Canada;(4) Crop Science Department, University of Guelph, Guelph, Canada |
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| Abstract: | A simple particle bombardment device was designed, constructed and shown to be efficient for the delivery of DNA into plant cells. High levels of transient  -glucuronidase expression were observed in alfalfa suspension-cultured cells and embryogenic soybean suspension-cultured cells. Expression of -glucuronidase in alfalfa suspension-cultured cells was used to optimize the bombardment conditions for the device. Transient gene expression in alfalfa was found to be dependent on the state of the target tissue, the size of particles employed, the helium pressure used to accelerate the particles and the distance travel led by the tungsten particles carrying DNA.Abbreviations 2,4-d
2,4-dichlorophenoxyacetic acid
- GUS
-glucuronidase
- MS
Murashige & Skoog (1962) medium |
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| Keywords: | transient gene expression -glucuronidase alfalfa soybean DNA transfer |
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-glucuronidase alfalfa soybean DNA transfer