Developmental and Environmental Effects on Sesquiterpene Lactones in Cultivated Arnica montana L. |
| |
Authors: | Milka Todorova Antoaneta Trendafilova Antonina Vitkova Maria Petrova Ely Zayova Daniela Antonova |
| |
Institution: | 1. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, BG‐1113 Sofia;2. Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, BG‐1113 Sofia;3. Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, BG‐1113 Sofia |
| |
Abstract: | The amount of sesquiterpene lactones and the lactone profile of Arnica montana L. in flowering and seed formation stages in vitro and in vivo propagated from seeds of German, Ukrainian, and Austrian origin and grown in two experimental fields were studied. It was found that in vitro propagated 2‐year plants in full flowering stage accumulated higher amount of lactones in comparison to in vivo propagated 3‐year plants and to the seed formation stage, respectively. Helenalins predominated in in vivo propagated 2‐year or in vitro propagated 3‐year plants. 2‐Methylbutyrate (2MeBu) was the principal ester in the samples with prevalence of helenalins, while isobutyrate (iBu) was the major one in the samples with predominance of 11,13‐dihydrohelenalins. The results revealed that the environmental conditions on Vitosha Mt. are more suitable for cultivation of A. montana giving higher content of lactones. |
| |
Keywords: | Arnica  montana Asteraceae Sesquiterpene lactones Helenalins 11 13‐Dihydrohelenalins Developmental and environmental factors |
|
|