Cell cycle analysis using the monoclonal antibody Ki-67 |
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| Authors: | H Isobe T Shimizu H Haneda S Mizuno A Ishiguro Y Araya M Harada H Miyamoto Y Kawakami |
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| Institution: | First Department of Internal Medicine, School of Medicine, Hokkaido University. |
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| Abstract: | We determined by flow cytometry the proportion of cells in cycle with the monoclonal antibody Ki-67 and also in S-phase after incorporation of bromodeoxyuridine (BrdUrd) with monoclonal antibody to BrdUrd. The monoclonal antibody Ki-67 was useful to detect a nuclear antigen present only in proliferating cells but not expressed in resting (Go) cells. Cell preparation to measure BrdUrd amount incorporated into cellular DNA was difficult but this anti-BrdUrd antibody was useful for measuring the rate of DNA synthesis and for the analysis of precious cell kinetics. These antibodies may provide useful information of cell kinetics. |
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