The oxidative metabolism of arachidonic acid by purified cytochromes P-450 |
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Authors: | Jorge Capdevila Linda Parkhill Nicholas Chacos Richard Okita Bettie Sue S Masters Ronald W Estabrook |
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Institution: | Department of Biochemistry Southwestern Medical School University of Texas Health Science Center at Dallas Dallas, Texas 75235 USA |
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Abstract: | Arachidonic acid is catalytically oxidized using either of two types of purified cytochrome P-450 reconstituted with the purified flavo-protein, NADPH-cytochrome P-450 reductase. The reaction is dependent on the presence of cytochrome P-450, NADPH, and oxygen. The patterns of products formed are unique for the type of cytochrome P-450 used. This suggests an enzyme-directed specificity of the site of attack on the unsaturated fatty acid by the hemeprotein. Additional experiments show a possible role for cytochrome b5 since the addition of purified cytochrome b5 enhances the rate of metabolism of arachidonic acid 2 to 3 fold. |
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