Participation of a cytochrome b5-like hemoprotein of outer mitochondrial membrane (OM cytochrome b) in NADH-semidehydroascorbic acid reductase activity of rat liver |
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| Authors: | Akio Ito Shin-ichi Hayashi Touho Yoshida |
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| Institution: | Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812, Japan |
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| Abstract: | The participation of a cytochrome b5-like hemoprotein of outer mitochondrial membrane (OM cytochrome b) in the NADH-semidehydroascorbate (SDA) reductase activity of rat liver was studied. NADH-SDA reductase activity was strongly inhibited by antibodies against OM cytochrome b and NADH-cytochrome b5 reductase, whereas no inhibition was caused by anti-cytochrome b5 antibody. NADH-SDA reductase exhibited the same distribution pattern as OM cytochrome b-mediated rotenone-insensitive NADH-cytochrome c reductase activity among various subcellular fractions and submitochondrial fractions. Both activities were localized in outer mitochondrial membrane. These observations suggest that OM cytochrome b-mediated rotenone-insensitive NADH-cytochrome c reductase system participates in the NADH-SDA reductase activity of rat liver. |
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| Keywords: | SDA semidehydroascorbate |
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