Increased synthesis of a low molecular weight protein in vincristine-resistant cells

A 19,000-dalton peptide (pI = 5.7) that is synthesized in increased amounts in vincristine-resistant Chinese hamster cells ($\text{DC-3F}\text{VCRd-5}$) has been identified by two-dimensional gel electrophoresis. Reduced amounts of the protein were present in a revertant line of $\text{DC-3F}\text{VCRd-5}$, and only trace amounts were detected in control DC-3F cells. A similar protein (M_r = 19,000; pI = 5.7) was also found in a vincristine-resistant mouse line. Two vincristine-resistant human neuroblastoma cell lines likewise contained elevated levels of a low molecular weight acidic protein. Increased biosynthesis of the 19,000-dalton polypeptide in $\text{DC-3F}\text{VCRd-5}$ cells coincides with the presence of a homogeneously staining region, HSR, on a metaphase chromosome.