Susceptibility of turbot (Scophthalmus maximus), coho salmon (Oncorhynchus kisutch, and rainbow trout of. my kiss) to strains of Vibrio anguillarum and their exotoxins |
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| Authors: | Ysabel Santos R Laixier Isabel Bandin J Lamas Alicia E Toranzo |
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| Institution: | Departamento de Microbiología y Parasitología, Facultad de Biología Universidad de Santiago, Santiago de Compostela, Spain.;Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal, St. Hyacinths, P. Q. Canada;Departamento de Biología Fundamental, Facultad de BiologíAa, Universidad de Santiago, Santiago de Compostela, Spain |
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| Abstract: | The susceptibility of turbot, coho salmon, and rainbow trout to strains of Vibrio anguillarum of serotypes 01 and 02 and their extracellular products (ECP) was investigated in order to clarify the role of exotoxins in the mechanism of virulence of both serotypes. All V. anguillarum isolates were virulent for trout, salmon, and turbot. Despite the origin of the strains tested, rainbow trout was the most susceptible fish species to experimentally induced vibriosis. Coho salmon and turbot did not differ significantly in their susceptibility to V. anguillarum live cells. In contrast, the ECP from Vibrio strains of serotypes 01 and 02 exhibited similar lethal dose for turbot, salmon, and trout (ranging from 4.52 to 7.32 μg protein/g fish). Therefore, differences in susceptibility to vibriosis are not completely due to a differential sensitivity of fish to the extracellular products of Vibrio strains. The ECP from 7 of 10 V. anguillarum strains possessed vascular permeability factors, and all the extracts displayed proteolytic, hemolytic and cytotoxic activities. All the biological activities of ECP were lost after heat treatment at 80° C/10 min. |
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